Starting XI with clubs
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| No | Name | Age | Club | Goal | Assist | Card | Sub. |
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| 1 | Petr Čech | 30 | Chelsea |
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| 2 | Theodor Gebre Selassie | 25 | Slovan Liberec |
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| 5 | Roman Hubník | 28 | Hertha |
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| 6 | Tomáš Sivok | 28 | Beşiktaş |
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| 3 | Michal Kadlec | 27 | Leverkusen |
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| 13 | Jaroslav Plašil | 30 | Bordeaux |
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52'
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| 19 | Petr Jiráček | 26 | Wolfsburg |
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Out: 76' |
| 9 | Jan Rezek | 30 | Anorthosis |
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Out: 46' |
| 10 | Tomáš Rosický | 31 | Arsenal |
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| 14 | Václav Pilař | 23 | Viktoria Plzeň |
52'
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| 15 | Milan Baroš | 30 | Galatasaray |
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Out: 85' |
| 17 | Tomáš Hübschman | 30 | Shakhtar Donetsk |
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In: 46' |
| 11 | Milan Petržela | 28 | Viktoria Plzeň |
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In: 76' |
| 21 | David Lafata | 30 | Jablonec |
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In: 85' |
| Coach | Michal Bílek | 47 |
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